Plastination of stained sections of the human brain.
نویسندگان
چکیده
A method is described which combines staining of human brain tissue with subsequent plastination of the sections. Staining of 1-4 mm thick frozen sections with astra blue or aldehydefuchsin provides a sharp contrast between white and grey matter. The plastination results in sections which are durable and convenient to handle. These preparations may be used for teaching purposes in neuroanatomy and neuropathology.
منابع مشابه
Plastination and Staining of Brain Slices Using Two Different Dehydration Methods
Introduction: Unstained formalin-fixed whole brain specimens and brain slices do not give satisfactory results for teaching neuroanatomy. In addition, difficulties in obtaining human brains for dissection have increased the demand for more durable brain specimens that have been obtained by the plastination technique. In the present study brain specimens were sliced, fixed and stained using...
متن کاملPlastination and Staining of Brain Slices Using Two Different Dehydration Methods
Introduction: Unstained formalin-fixed whole brain specimens and brain slices do not give satisfactory results for teaching neuroanatomy. In addition, difficulties in obtaining human brains for dissection have increased the demand for more durable brain specimens that have been obtained by the plastination technique. In the present study brain specimens were sliced, fixed and stained using the ...
متن کاملA New Method of Brain Plastination
Introduction: Plastination is a unique technique for preservation of biological specimens used for teaching purposes. The protocol of flexible sheet plastination includes fixation, slicing, dehydration, force impregnation, casting, and curing (Hajian, Rabiei, Fatollahpour & Esfandiary, 2008). The procedure is done by using P87 flexible unsaturated polyester resin and provides heavy, gross, frag...
متن کاملSynaptophysin-Positive Neurons in External And Extreme Capsules in Human Brain
Purpose: Externaland extreme capsules are parts of cerebral white matter, and accordingto the classic knowledge they must have no neuronal cell bodies.This researchis designedto find neurons in the external and extreme capsules, and to determine that these neurons are functional or aberrant. Materitais and Methods: Ten adult normal human brains from both sexes were studied using 15,µm serial c...
متن کاملOriginal Research Article
Division of Biomedical Sciences (Anatomy) St George's, University of London London, UK ABSTRACT: The standard method for plastination with Biodur TM S10/S3 involves lowtemperature dehydration in a volatile intermediary solvent followed by forced impregnation under vacuum at -15°C. However, some institutions have been reluctant to install low-temperature impregnation equipment because of health ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Anatomischer Anzeiger
دوره 170 5 شماره
صفحات -
تاریخ انتشار 1990